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They were transfected for 3 h in Opti-MEM I (Invitrogen corp., carlsbad, CA, USA), and then the medium was changed to RPMI 1640 with 5% fetal bovine serum and they were incubated for further 33 h

Human breast milk enhances intestinal mucosal barrier function and innate immunity in a healthy pediatric human enteroid model

Barcodes were added at the 5 terminus of the forward primer to allow for sample multiplexing during sequencing 55

Peer review Peer review information Nature Biomedical Engineering thanks the anonymous reviewers for their contribution to the peer review of this work

Evaluation of malondialdehyde level, total oxidant/antioxidant status and oxidative stress index in colorectal cancer patients