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Viral infection For lentiviral production, HEK293T cells were co-transfected with 4 g 8.91, 1 g pVSVG, 5 g of lentivector (shRNA targeting SHMT2 : CCAGAATTTGTAGCTGAGATT, and ATF4 : GCGAGTGTAAGGAGCTAGAAA, obtained from RNA Technology platform and Gene manipulating core, Academia Sinica) by using 30 L of Turbofect (Thermo, R0533) in 1 mL of Opti-MEM (Gibco, 31985070) according to the manufacturers instructions

doi:10.1016/j.soard.2021.04.009 Balen AH, Morley LC, Misso M, et al

The natural cycle also slows down due to environmental factors

P53-dependent glutamine usage determines susceptibility to oxidative stress in radioresistant head and neck cancer cells

Importantly, it can replace some pharmaceuticals with severe contraindications for elderly patients, offering better tolerance