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To compare simulated concentration time-courses with measured time-course data, we include the unknown concentration scale of fluorescence microscopy measurements and possible background fluorescence into an observation function

For example, in the peroxisome where verylongchain FAs are preferentially metabolized, carnitine shuttles the fatty acyl end products of beta oxidation between the peroxisome and the mitochondria (Steiber et al., 2004)

Some practitioners combine both approaches

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Therefore, high GSH/GSSG ratios in cancer cells can also be explained by increased flux through the pentose phosphate pathway (PPP), which produces NADPH (Li et al., 2014a