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[The Working Group noted that the latter study was limited by the lack of quantitative or semiquantitative evaluation.] After a 24-hour at 320 g/mL (IC 25 ) or 480 g/mL (IC 50 ), aspartame induced phosphorylation of histone H2AX (H2AX), a biomarker of DNA double-strand breaks, in HepG2 (human hepatoblastoma) cells (Qu et al., 2019)