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Mannervik B, Danielson UH

Bacterial pellets were resuspended in GST-binding buffer (25 mM Tris-HCl pH 7.5, 150 mM NaCl, 1 mM EDTA) containing protease inhibitors

So novel PEGylated bilirubin-encapsulated CaWO4 nanoparticles(PEG-BR/CWO NPs) were reported by Pizzuti et al (232)

In addition, we performed site-directed mutagenesis and biochemical analysis based on the structural information, which revealed that the Cys20 and Lys8 residues are important for enzymatic activity