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thetaiotaomicron (100 l overnight culture) in 10 ml of pre-reduced low-glucose Dulbeccos Modified Eagles medium (Gibco Life Technologies, Grand Island, NY, USA) supplemented with cyanocobalamin at 0 ppm, 0.01 ppm, and 15 ppm, which was subsequently incubated for 6 h

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This, in turn, activates the receptor molecules and triggers the downstream cascade signaling pathway