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Finally, staining was performed with 3,3-diaminobenzidine (DAB, Sigma, D12384) substrate and counterstained with hematoxylin for nuclear differentiation

In mature astrocytes differentiated from human induced pluripotent stem cells (iPSC)-derived astrocyte progenitors, ketamine can exert rapid antidepressant effects through the activation of amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA) glutamate receptors, and estrogen enhances this effect of ketamine by increasing the gene expression of AMPA receptor subunits

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Niacinamide, Collagen Extract, Glutathione, Adenosine, Allantoin, Centella Asiatica Extract, Amino Acid Complex, Chrysanthemum Indicum Flower Extract, Curcuma Longa Root Extract, Hibiscus Sabdariffa Flower Extract, Tocopherol

The defendants remain free to challenge those opinions, introduce their own experts, cross-examine the plaintiffs witnesses, and argue that the scientific evidence does not establish causation