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Acyl-Coenzyme A synthase long chain family member 4 (ACSL4) is thought to be a specific biomarker and driver of ferroptosis as it is a key enzyme involved in fatty acid metabolism

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Blood samples are collected at time intervals of 0.5, 1, 2, 4, 6, 8, 10, 12 and 24 hrs and the content of GHK-Cu was estimated

The first strand synthesis reaction can then be used as a template for PCR amplification of the desired 5 end using a primer specific to the ligated RNA oligonucleotide and a primer specific to the known sequence of the apoptosis related of interest