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The precipitates derived from each supernatant were dissolved in Tris buffer (2 mM EDTA/250 mM Tris-HCl) at pH 8.5, reduced with 0.67 M dithiothreitol, alkylated with 1.4 M iodoacetamide and digested with trypsin

Due to the high yield, give it a few extra minutes for the lyophilised solid to dissolve into a clear, slightly blue-tinted liquid

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