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The metabolic profiling of TNBC patient serum, however, shows significant differences from MDA-MB-468 culture media, suggesting that in vitro models may not fully replicate systemic metabolic changes in patients

Nutrients 15 , 3411 (2023)

Western blotting Cells and tissues were lysed in RIPA buffer (beyotime, China) containing proteinase inhibitor and phosphatase inhibitor (Thermo Fisher, United States) to extract total proteins and analyzed as previously described [20]

142 Furthermore, histone methylation and acetylation are closely associated with BCG-induced host-trained immunity against Mtb

Following the incubation, we removed the medium and washed the cells with PBS and with that, all the residuals away