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In contrast to the almost exclusive localization of AsGGT2 N100 -GFP in the vacuole ( Figure 7B ), the green fluorescence signals from AsGGT2 N300 -GFP and AsGGT2 Full -GFP were detected both in the vacuole and cytosol (data not shown), suggesting that a part of AsGGT2 N300 -GFP and AsGGT2 Full -GFP polypeptides was not properly processed and/or assembled and thus was not sorted to the vacuole in the heterologous expression system we used

doi: 10.3389/fnut.2022.1082500 89 LuongKVQNguynLTH

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The loop between TM1 and TM2 forms a lid over the active site in LTC4S and would have to fold away to allow for an extended conformation of GSH