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To this end, we cultured regular LAPC4-AD cells long-term (4 months) in charcoal dextran stripped serum (CDSS) medium to develop castration-resistant LAPC4 (LAPC4-CR or LAPC4-AI) cells, which were subsequently exposed to either 20 M or 100 M Enza-containing CDSS medium for 1 month resulting in the LAPC4-Enza(20)-R or LAPC4-Enza(20)-R models (supplementary Fig

Denaturating agents, such as urea or guanidinium hydrochloride may be used to solubilize peptides which tend to aggregate

-20C, 1 month (protect from light, stored under nitrogen) Solvent & Solubility In Vitro : DMSO : 100 mg/mL ( 184.98 mM