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From a vaccine technology development point of view, this is an exciting time and novel technologies and approaches are poised to make a clinical impact for the first time

Membranes were blocked with 5% BSA in PBS-Tween-20 for 1 h at room temperature and incubated overnight at 4 C with one of the following primary antibodies: rabbit polyclonal anti-BAX (Cell Signaling Technology), rabbit polyclonal anti-BNIP3 (Cell Signaling Technology), or mouse monoclonal anti-FLAG (clone M2)

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Meanwhile, the inflammatory cytokines IL-18 and IL-1 may serve as the markers of the severity of AP patients (Janiak et al., 2015)

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