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3D Co-Culture Model Matrigel basement membrane matrix (Corning, Corning, NY, USA) was thawed overnight in a 4 C refrigerator

This biological limitation has driven research into regenerative medicine strategies designed to: Restore disc hydration Support extracellular matrix synthesis Improve disc height and structural integrity Modulate inflammatory microenvironments Structural Metrics That Can Be Objectively Measured Another reason the intervertebral disc is a focus of regenerative research is the availability of quantifiable imaging and functional metrics , such as: Disc height measured on MRI Herniation size and morphology Alignment changes (lordosis, scoliosis) Functional indices such as the Oswestry Disability Index (ODI) These parameters allow researchers to objectively evaluate structural and functional changes following investigational therapies, as demonstrated in recent clinical case reports

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Together, they may cover more aspects of the weight-loss process

Use only sterile, single-use syringes and needles for both the addition of the diluent and the extraction of the reconstituted solution