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Additional causes of liver damage include alcoholic liver damage, nonalcoholic fatty liver disease (NAFLD) due to fat accumulation, and drug-induced liver injury (Figure 1)

The GSH redox cycle is a major source of protection against mild oxidative stress with the GPx, antioxidant enzyme that oxidizes GSH to GSSG to protect cells against the proliferation of reactive oxygen species (ROS) or reactive nitrogen species (RNS), sparing them from oxidative damage, while catalase is becoming increasingly important in protecting against severe oxidative stress [3, 6, 10]

Add 10 ml of the secondary antibody solution to the membrane and leave it for 1 hrs