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Then, 500 L of PBS was applied to the tube, and the supernatant was removed again via centrifugation (1,300 rpm for 4 min) and vacuuming

doi: 10.1111/j.1365-2052.2006.01436.x

Relative viral titer was determined by infecting cells with serially diluted volumes of virus-containing medium and selecting using appropriate selection reagents

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