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The next day, the membranes were washed three times with TBST and then incubated with anti-rabbit IgG conjugated with IRDye 800CW (LI-COR Biosciences, Lincoln, Nebraska, USA) for 1 h at RT

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To be sure, later national media stories inspired other cities, towns and school systems to step up their testing for lead and provided a reminder of how toxic many neighborhoods are across this country