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2.4.8 DNA extraction from homogenized tissue Total DNA was extracted from intestinal mucosa and lymph nodes as previously described ( 2.4.9 qPCR for identification of MAP DNA from tissue and fecal samples To detect MAP genomes in DNA samples, a two-duplex real-time qPCR assay targeting IS900 and F57 was employed ( 2.5 Ex-vivo trial to evaluate the functionality of effector immune cells in killing wild-type MAP 2.5.1 Preparation of antigen presenting cells to prime effector immune cells The ex vivo trial was designed and conducted as previously described ( Figure 1D )

M.BolboacS

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Tong et al (101) found that on the basis of the antifibrotic properties of miR-29, AS-IV + FA (AF) can use miR-29b as a drug target to induce Smad3 dephosphorylation further and alleviate oxidative stress and PF by triggering nuclear factor erythroid 2-related factor 2 (Nrf2) activation and interfering with TGF-1/Smad3 signaling

The mTOR pathway negatively controls ATM by up-regulating miRNAs