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The SIF was a commercially available product formulated according to pharmacopeial guidelines, consisting primarily of potassium dihydrogen phosphate (KHPO), sodium hydroxide for pH adjustment, and purified water, without digestive enzymes

The relative quantitative method of 2 Ct was employed to calculate the target gene expression levels, which were then analyzed in Excel

Moreover, overexpression of GPX4 with adeno-associated virus significantly attenuated neuronal cell death and improved mice behavior (Zhang et al., 2018)

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Priolo C, Tang D, Brahamandan M, Benassi B, Sicinska E, Ogino S et al