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The absorbance at a wavelength of 420 nm was continually measured for a duration of 2 min, with measurements obtained during the time interval of 1 to 3 min

Specifically, the investigation revealed that this antioxidant regimen preserves Complex IV activity, thereby maintaining optimal electron transport chain function and ATP biosynthesis

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Future studies should prioritize defining the precise abundance threshold of Akkermansia muciniphila that balances its beneficial and deleterious effects on the intestinal barrier, investigating the molecular mediators (e.g., specific metabolites or surface proteins) that underpin its dual regulatory role, and developing targeted interventions (e.g., precision probiotics or microbiota modulators) to sustain its homeostatic levelswith the goal of ultimately mitigating OA progression via the gut-joint axis

Ferroptosis has been implicated in the pathogenesis of various vascular-related diseases, including (AS), stroke, and Ischemia/Reperfusion Injury (Fang et al., 2023