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Briefly, the gene encoding the OsDHAR sequence (residues 2203) was amplified by PCR using Ex Taq polymerase (TaKaRa Bio Inc., Shiga, Japan) with forward and reverse primers for a cDNA template (Table S1)

[DOI] [PMC free article] [PubMed] [Google Scholar] 227.Vui V., Grabe M., Trchounian A., Arsi A

Nature 418:244251 Hebert CG, Valdes JJ, Bentley WE (2008) Beyond silencingengineering applications of RNA interference and antisense technology for altering cellular phenotype