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Disruption of NADPH production compromises GSH regeneration, limits GPX4-dependent detoxification of lipid peroxides, and can promote ferroptosis even in the absence of impaired GSH synthesis 152

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This is majorly done when too much glutathione is used without mixing it with the right ingredients to balance the antioxidant

The organic layer was collected and evaporated to dryness under nitrogen flow

Glycine biosensor characterisation Once it had been determined that the transformation was successful, it had to be shown that glycine would result in RFP production