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The isolated mitochondria were introduced to the oxygraphy chambers containing 2 mL of Buffer Z [105 mM MES potassium salt, 30 mM potassium chloride (KCl), 10 mM monopotassium phosphate (KH 2 PO 4 ), 5 mM magnesium chloride (MgCl 2 ), and 0.5 mg/mL BSA]

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