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Regarding the effects of zinc on obesity-related inflammation and its complications, zinc supplementation combined or not with restricted caloric diets has showed to modify inflammatory markers, reducing the levels of IL-6 and C-reactive protein (CRP) in obese individuals (100, 151)

Liposome flotation assay For the liposome flotation assay, 3.6 g of recombinant -SNAP (WT or M105I) was incubated with LUVs (6.25 mg/ml) in 40 l of NB buffer at 37 C for 5 min, following the protocol described by Tronchere and Boal with some modifications 66

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