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The genome-wide CRISPR screen was performed in HEK293 cells using the Brunello library 23 encompassing ~77,000 guide RNAs (sgRNAs) against ~19,000 human genes (4 sgRNas per gene) (Fig

Additionally, we observed increases in metabolites related to amino acid metabolism and biosynthesis, such as proline, L-Ala--D-glu-meso-diaminopimelic acid, 2-amino-2-butenonic acid, and N2-succinyl-L-arginine

Seiring berjalannya waktu, kadar glutathione akan mengalami penurunan karena paparan radikal bebas, stress, serta bertambahnya usia