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While your body produces free radicals naturally through metabolism, their production dramatically increases due to environmental factors like pollution, UV radiation, cigarette smoke, poor diet, and intense exercise

To this end, a 96-well Nunc Maxisorp plate (Thermo Fisher Scientific) was coated with 5 g/mL human CEA-Fc chimera (Genscript, Piscataway, NJ) or HER2 extracellular domain (EMP Genetech, Ingolstadt, Germany) in PBS (4 mM KH 2 PO 4 , 16 mM Na 2 HPO 4 , 115 mM NaCl, pH 7.4) at 4 C overnight and blocked with 3 % (w/v) bovine serum albumin (BSA) in PBS/T (PBS supplemented with 0.1 % (v/v) Tween-20) for 2 h at room temperature

10.1007/s11104-005-3900-1 143 RichauK

Sperm mitochondrial DNA deletion in Iranian infertiles with asthenozoospermia

typhi (Yadav et al., 2016)