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DHFR is a key enzyme in the process of thymidylate synthesis, catalyzing the folate reduction to THF in two steps: during the first step, folates are reduced to dihydrofolate (DHF), which are further reduced to THF (Neradil et al

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The percentage of late apoptosis in THJ-16 cells increased from 5.49% for untreated cells to 63.43% for treated cells, while the corresponding percentage in CAL-62 cells increased from 16.72 to 69.88%

After incubation, 3Ci of [methyl- 3 H] thymidine (Moravek Biochemicals