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Daolin Tang, [email protected] This article was submitted to Neuro-Oncology and Neurosurgical Oncology, a section of the journal Frontiers in Oncology Disclaimer All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers

At 36 h post-transfection, cells were selected with puromycin and verified in a T7E1 assay

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Clean the injection site with alcohol, pinch the skin to create a fold of tissue, insert the needle at a 45-90 degree angle, inject slowly, and withdraw the needle

Notably, substantial heterogeneity was appreciated between studies analyzed