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Wang F, Zhao W, Gao Y, Zhou J, Li H, Zhang G, et al

Fresh stock solutions of NADPH, GSH, GR and GSSCys or HEDS were prepared in assay buffer containing 0.1 M Tris/HCl, 1 mM EDTA, pH 8.0 before each experiment

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These results suggest that the cysteine residues of TAT are involved in the formation of intramolecular disulfide bonds