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Rather, the majority of orally ingested l-carnitine in mice produced a previously unappreciated intermediary metabolite, -butyrobetaine (BB), in a gut microbiotadependent step that occurred at a site anatomically proximal to, and at a rate 1,000-fold higher than the rate of the formation of TMA (26)

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KosinskiMBaylissMSBjornerJBWareJEJrGarberWHBatenhorstAet al

After that, we washed the blocking buffer with PBS-T and added the primary antibody for 8-OH-dG (GeneTex, GTX 35250) [1:200] overnight at 4 C