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Description

Total RNA was quantified by using the NanoDrop ND-1000A UVVIS spectrophotometer (Thermo Scientific, Wilmington, DE, USA) at an OD of 260 nm, and its purity was assessed by determining the OD260/OD280 ratio

Both mechanisms act on vascular smooth muscle, precapillary sphincters, and pericytes, with particularly strong effects at the level of precapillaries and capillaries, highlighting the key role of these microvascular segments in CVR ( Astrocytes also contribute to CVR through CO 2 -dependent intracellular Ca 2+ signaling