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Ni-NTA beads were equilibrated in Buffer A and added to the cleared supernatant

Differentiation of B cells in the nonlymphoid tissue of the synovial membrane of patients with rheumatoid arthritis

Tsuchiya Y, Kobayakawa S, Tsuji A, Tochikubo T

Neutrophils were then stimulated with PVL (150 nmol/L) at room temperature for 45 min, or with 100 nmol/L PMA for 2 h at 37 C, 5%CO 2 , followed by addition of Sytox green (1 mol/L) and Hoechst33342 (5 g/mL) for a further 15 min in the dark