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Adv Biol (Weinh) 8:e2300110

Purified fusion GST X protein, purified Y protein, and glutathione GSH magnetic beads that can specifically bind to GST are mixed together and incubated for a certain period of time

In fed-batch fermentation, the #14 mutant achieved 1.98 g/L of glutathione using molasses and CSL as carbon and nitrogen sources, comparable to yields from expensive complex media containing peptone and yeast extract

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3,4-Methylenedioxymethamphetamine induces differential regulation of tryptophan hydroxylase 2 protein and mRNA levels in the rat dorsal raphe nucleus