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Ambion/Life Technologies (Grand Island, NY, USA)) were transfected using lipofectamine 2000 in OPTIMEM according to the manufacturers instructions

ISCs are involved in electron transport, substrate binding, enzymatic catalysis and play critical roles in maintaining genomic stability since several DNA polymerases and helicases require interaction with ISCs for proper functioning

Moreover, GPX4 degradation can also result in ferroptotic cell death

Hydrogel texture adheres well for effective ingredient delivery

For the reconstitution experiment, silent point mutations were introduced into the ABCC1 cDNA using PCR mutagenesis to avoid targeting by ABCC1 sgRNAs and the product was cloned into a pMSCV-PGK-BlastR retroviral expression vector using Gateway cloning