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Another significant component is the mast cell degranulation peptide (MCD), also known as peptide 401, which accounts for about 2% to 3% of BVs weight (56)

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After incubation at 4 C for 30 min, the reaction mixtures were added to 100 L of 50% (v/v) Ni-NTA superflow resin slurry that was pre-equilibrated with buffer containing 50 mM BisTris, pH 6.8, 100 mM NaCl, 25 mM imidazole, and 10% glycerol

However, this association was no longer significant after adjusting for offspring BMI, indicating that body weight mediates the relationship between GDM exposure and epigenetic aging [166]