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Transduction of ATP7B-KO cells with each pool was performed according to the manufacturers instructions with a lentiviral MOI of 0.3 to ensure the integration of not more than one shRNA per cell

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Avoid aggressive shaking, as excessive agitation may affect product stability

Stimulation of matrix synthesis In fibroblast cultures, GHK-Cu increases the synthesis of the major structural molecules of the extracellular matrix: collagen, elastin, and the glycosaminoglycan/proteoglycan ground substance (including dermatan sulfate and other glycosaminoglycans)

Try cycling (8 weeks on, 4 weeks off) versus continuous