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Description

R., Genuardi, M., and Zammarchi, E

It is a standard research tool in cell biology and endocrinological research

Approximately 50 mg of frozen cecal content was added to 0.1 mm glass bead-containing tubes (PowerBead Tubes, Qiagen) prefilled with 300 l RLT buffer (RNeasy mini kit, Qiagen) supplemented with -mercaptoethanol (10 l/ml, Sigma-Aldrich) and 1 ml TRIzol (Invitrogen)