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It was indirectly confirmed by the result of patch assay that W303-1b/gsh1/pro1 with Pro1 overexpression construct performed a relatively stronger H 2 O 2 tolerance than W303-1b/gsh1/proB and W303-1b/gsh1 (Fig

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6g).To further interrogate the role of ADH5 in maintaining the GSH:GSSG ratio upon FA stress, we incorporated the cytoplasmic version of the reporter Grx1-roGFP2 35 in WT and ADH5 HCT116 cells

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