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The details of the chemistry behind the reaction are available from vendor product literature, but briefly, the assay utilizes two bead types, a glutathione-coated acceptor bead which binds recombinant human FcR-Glutathione-s-transferase (FcR-GST), and a streptavidin donor bead which contains a photosensitizer and binds to biotinylated human IgG1

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Blood samples were obtained at the start and end of the 2-week trial, during which patients were randomly assigned to receive either active supplementation (2 softgels/day corresponding to ALA 600 mg/day) or no supplementation at all