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Do not apply open wounds

Wang L, Li S, Zhang Y

Their diversity reflects the complexity of the proteome: mammals encode over 600 E3 ligases, far exceeding the number of E1 and E2 enzymes

At 1.5 h of incubation, 50 l supernatant was removed to a new 96-well plate for glycerol analysis and a fresh 50 l stimulation buffer was added before placing the plate back in the incubator