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Screening for antigen specific T cells in 3 donors reveals that PHOX2B TCRs have minimal target binding, suggesting that PHOX2B self-antigen is immunogenically silent and warrants targeting using synthetic scFv receptors

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Huber W (1981) Orgotein(bovine Cu-Zn superoxide dismutase), an anti-inflammatory protein drug: discovery, toxicology and pharmacology

For each of the samples, 1 mg of total protein extract was incubated with -HA beads in TPA buffer (30 mM tris pH 7.5, 100 mM NaCl, 5 mM MgCl 2 , 2 mM DTT, 10% glycerol, 0.01% IPGAL and supplemented with protease inhibitors) for two hours at 4 C with rotation