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However, none of the aforementioned studies provided experimental evidence for antiviral activity of phenformin in cell-based or in vivo infection models

To construct 35Spro::DRM2 , 35Spro::SUVH1-6Myc , and gSUVH1-6Myc , the coding sequences of DRM2 and SUVH1 , and a 3.3 kb genomic region of SUVH1 , including the promoter and gene body, were amplified from Col-0 and cloned into the pSuper1300-Myc plasmid, respectively

Methods: Serum samples from a cross-sectional cohort of 562 untreated HBeAg-negative patients (median age 38 y, 204 males) were tested for routine diagnostic markers: HBV DNA (Roche TaqMan, IU/ml), quantHBsAg (Abbott Architect, IU/ml), biochemical (ALT, IU/L) and further tests were performed for the biomarkers: HBcrAg (CLEIA Fujirebio, log 10 U/ml), pgRNA (Abbott Diagnostics dual-target real-time-PCR assay, LLQD=0.4 log 10 U/ml), CXCL10 and sPD-1 serum levels by ELISA [pg/ml]