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pGEX series expression plasmid Plasmid size: 4969bp Plasmid label: N-GST, N-thrombin Prokaryotic resistance: ampicillin Amp Cloned strain: DH5 alpha Culture conditions: 37 centigrade, aerobic, LB Expression host: Escherichia coli Inducement: IPTG or lactose and its analogues 5'sequencing primers: pGEX5: GGGCTGGCAAGCCACGTTTGGTG 3'sequencing primers: pGEX3: CCGGGAGCTGCATGTGTCAGAGG Note: the recombinant protein can be purified by GST affinity column Use:pGEX Plasmid pGEX-4T-1 Description PGEX-4T-1 is a E.coli expression plasmid pGEX-4T-1 Sequence LOCUS Exported 4969 bp ds-DNA circular SYN 03-8-2015 DEFINITION synthetic circular DNA KEYWORDS pGEX-4T-1 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4969) TITLE Direct Submission JOURNAL Exported 2015-8-3 FEATURES Location/Qualifiers source 1..4969 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 183..211 /note="tac promoter" /note="strong E

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