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Since only a subset of Mettl9 Deg expressed also LCS-HA at DIV5 (probably due to construct silencing) microscopy acquisition was performed on fields of view displaying LCS-HA positive cells (in Triton samples), and randomly in the digitonin samples, as the latter did not have any detectable signal in the 488 channel (LCS-HA)

Such a method in dried blood spots could also facilitate testing B12 status in large-scale surveys and in remote populations (Schroder et al., 2014)

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