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Free radicals can cause many types of damage known collectively as oxidative stress when they react with cellular components, especially the cell membranes

In its fully oxidized state, CoQ 10 accepts electrons from NADH-ubiquinone reductase (Complex I) and succinate-Q oxidoreductase (Complex II), and transfers them to Complex III

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For membrane and cytosolic subcellular fractionation, post-nuclear homogenates prepared in detergent-free buffer were ultracentrifuged at 150,000 g for 45 min at 4 C