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GAPDH primers were: Sense, 5-GGTCGTATTGGGCGCCTGGTC-3

10.1016/j.fsi.2023.109135 38 MarshallS

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This allows the cells to produce recombinant proteins with complex, terminally sialylated N-glycans, resulting in mammalian-like glycosylation and higher overall glycosylation levels compared to proteins produced by the original Sf9 or High Five cells (Legardinier et al., 2005