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After 30 min, the lysate was clarified by centrifugation (1500 g ), filtered (0.22 m filter), aliquoted and the E1E2-containing lysates were stored at 80 C

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In summary, how to specifically inhibit the amino acid metabolism of tumor cells and immunosuppressive cells in the microenvironment, and enhance the amino acid metabolism of anti-tumor immune cells such as CD8 + T cells, is an urgent problem to be solved