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For longer storage, freeze single-use aliquots at minus 20C and avoid repeated freeze-thaw cycles, which break the molecule down

Dissociated hippocampal neurons were treated with Hinge (10 " ' 2 M), HGF, Dihexa (10 "12 M) or HGF (lOng/ml) for 5 days after at which time mEPSCs were recorded in the absence of action potentials