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The -arrestin1 siRNA (5-CCAGCUCAACAUUCUGCAATT-3) and -arrestin2 siRNA (5-GCUUGUGGAGUAGACUUUGTT-3) purchased from GenePharma (Shanghai, China) were transfected into astrocytes using Lipofectamine 3000 reagent (Invitrogen, L3000015) according to the manufacturers protocol

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Then, the samples were dehydrated using ethyl alcohol and replaced by acetone

This patient's lack of cutaneous melanomas and presence of an endocrine neoplasm triad make a cell cycle regulation gene mutation unlikely

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