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2.6 Total antioxidant capacity and glutathione content Tissue samples (g) were homogenized in an ice bath using extraction solution at a 1:10 volume ratio (approximately 0.1 g tissue weighed and mixed with 1 mL pre-chilled extraction solution) ( 2.7 Cd content determination Plant samples were dried to constant weight after collection by organ, pulverized using a grinder, sieved through a 100 mesh screen, and then digested using the hot plate method before measurement by inductively coupled plasma mass spectrometry (ICP-MS) ( 2.8 Flavonoid and phenolic acid content Cinnamic acid and p-coumaric acid content in samples were determined by high performance liquid chromatography (HPLC) following the method of 1 fresh weight (FW), while kaempferol and quercetin content were measured using the method of 1 FW

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No, BPC-157 is not safe for long-term use because controlled human clinical data on safety, dosage, and long-term effects remain limited

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Can J For Res 44:554561